Journal: Scientific Reports

Article Title: Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)

doi: 10.1038/s41598-024-55241-z

Figure Lengend Snippet: Detection of cytochrome b gene from human mitochondrial DNA using LAMP. ( A ) shows the signal fluorescence intensity over time, while ( B ) shows the melting profiles of the DNA amplicons at the end of the reaction. As expected, the time to detection of the amplicon was dependent on the initial template concentration in ( A ), while the presence of a single peak in ( B ) indicates a single concatenated amplicon is formed. DNA quantities per reaction (1000, 360, 180 or 36 pg in 12.5 µL) are colour-coded, where solid lines represent the mean and the shaded areas represent the 95% confidence interval for signal (N = 2/3). NTC = no template control.

Article Snippet: The reasons for this could include that the previous report used a proprietary polymerase and buffer mix for LAMP (Optigene; cat. no. 004LNL) and that DNA sample preparation involved crude basification of the sample.

Techniques: Fluorescence, Amplification, Concentration Assay

Journal: Scientific Reports

Article Title: Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)

doi: 10.1038/s41598-024-55241-z

Figure Lengend Snippet: The effect of various inhibitors (hematin, humic acid and tannic acid) on real-time LAMP product detection and end point analysis on agarose gels. ( A ) shows the signal fluorescence intensity over time. Inhibitor concentrations are colour-coded per graph, where solid lines represent the mean and the shaded areas represent the 95% confidence interval for signal (N = 3). Arrows indicate the trend upon increasing inhibitor concentration for either the time to detection of the amplicon above the background. For tabular time to detection values see Supplementary Fig. . ( B ) shows amplicons run on agarose gels post-stained with ViSafe Red Gel Stain. Gels are loaded with increasing inhibitor concentrations as specified in A . 0 = positive control. L = 100 bp DNA base pair ladder, NTC = no template control.

Article Snippet: The reasons for this could include that the previous report used a proprietary polymerase and buffer mix for LAMP (Optigene; cat. no. 004LNL) and that DNA sample preparation involved crude basification of the sample.

Techniques: Fluorescence, Concentration Assay, Amplification, Staining, Positive Control

Journal: Scientific Reports

Article Title: Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)

doi: 10.1038/s41598-024-55241-z

Figure Lengend Snippet: The effect of various inhibitors (bile salts, CaCl , IgG and urea) on real-time LAMP product detection. ( A ) shows the signal fluorescence intensity over time. Inhibitor concentrations are colour-coded per graph, where solid lines represent the mean and the shaded areas represent the 95% confidence interval for signal (N = 2/3). Arrows indicate the trend upon increasing inhibitor concentration on the time to detection of the amplicon above the background. For tabular time to detection values see Supplementary Fig. . ( B ) shows the melting profiles of the DNA amplicons at the end of the reaction. Arrows indicate the trend upon increasing inhibitor concentration on the T m (peak maxima) of the amplicons. ( C ) amplicons run on agarose gels post-stained with ViSafe Red Gel Stain. Gels are loaded with increasing inhibitor concentrations as specified in ( A ). 0 = positive control. L = 100 bp DNA base pair ladder, NTC = no template control.

Article Snippet: The reasons for this could include that the previous report used a proprietary polymerase and buffer mix for LAMP (Optigene; cat. no. 004LNL) and that DNA sample preparation involved crude basification of the sample.

Techniques: Fluorescence, Concentration Assay, Amplification, Staining, Positive Control